Part:BBa_K607035

pUC18 replication origin

BBa_K607035 is a pUC18 origin of replication.

Usage and Biology

BBa_I50032 yields vectors with an expected copy number of 30-40 per cell.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Note: This part did not have a reference sequence. A reference sequence has since been added based on the part's documentation; a basic part consisting of the origin of replication from the pUC18 vector (pMB1). The sequence may contain additional bases not included in the function of the origin. - iGEM HQ

Functional Parameters: Austin_UTexas

BBa_K607035 parameters

Burden Imposed by this Part:

Burden Value: -2.9 ± 3.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.